What is klenow fragment?

Klenow fragment is a truncated version of DNA polymerase I isolated from E. coli. It is commonly used in molecular biology research for various applications including DNA sequencing, site-directed mutagenesis, and DNA labeling.

The Klenow fragment lacks the 5' to 3' exonuclease domain of DNA polymerase I, but still retains its 5' to 3' polymerase activity. This makes it particularly useful for filling in gaps in DNA molecules and for synthesizing complementary DNA strands for cloning purposes.

Klenow fragment is also used in the Klenow fragment DNA polymerase chain reaction (KF-PCR), a modified version of PCR that uses the enzyme for DNA amplification.

Overall, Klenow fragment is a valuable tool in molecular biology research due to its specific properties and versatility in various laboratory techniques.